Liang, S., and Crutcher, K. A. (1993). Movement of embryonic chick sympathetic neurons on laminin in vitro is preceded by neurite extension. Journal of Neuroscience Research, 36, (6):607-20.
Abstract
Chick sympathetic neurons (E-9) are capable of moving on a laminin
substrate but not on more adhesive substrates in vitro. The effect
of laminin is dose-dependent and reduced by the addition of
anti-laminin antibodies, whereas soluble laminin does not stimulate
movement. The onset of neuronal movement is preceded by, and highly
correlated with, the onset of neurite formation. The addition of 1,2
dioctanoyl-sn-glycerol (DAG), a stimulator or protein kinase C that
has been shown to inhibit neurite outgrowth, was found to delay both
process formation and neuronal movement but did not affect the
correlation between these two measures. These results support the
conclusion that laminin stimulates primary neuronal movement in
vitro and suggest that the mechanism underlying movement involves
process formation followed by "towing" of the cell body by the
advancing process. The similarities of this in vitro behavior to
that observed in vivo suggest that similar mechanisms may underlie
neuronal movement in the developing nervous system as suggested by
Morest (Z Anat Entwicklungsgesch 130:265-305, 1970) and Liesi (EMBO
J 4:1163-1170, 1985; Exp Neurol 117:103-113, 1992).
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